How would one work out the Tm value for a single primer? What is the Tm value for this primer sequence? 5'-GCCAGTCAAACAGTCAGTAA-3' B. ~47oC C. ~50oC D. ~53oC E. ~56oC : r/a:t5_318cy
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SOLVED: The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal and depends directly On iength and composition of the primer(s) An annealing temperature 5"€
SOLVED: Use the Wallace Rule equation to calculate the Tm of each of the primers you designed. Tm = 2(A+T) + 4(G+C) Left primer: 5' CCTGGACACTGTAC 3' Right primer: 5' AATGTATGAACCTG 3'
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Solved Calculate the melting temperature (Tm) for the 4 | Chegg.com
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SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule" This equation is: Tm = 2(A+T) + 4(G+C) Where A = # of
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